BM-MSCs are bone marrow stem cells, bone marrow hematopoietic stem cells (HSC) is not only mechanical support role, but also secrete a variety of growth factors (such as IL-6, IL-11, LIF, M-CSF and SCF, etc.) to support hematopoiesis.
MSC currently used methods include separation of whole bone marrow and density gradient centrifugation method that is based on whole bone marrow stem cells in low serum medium planted with adherent edge features, the medium was changed regularly adherent cells are not removed, so as to achieve purification and expansion MSC additional purposes. Density gradient centrifugation of the cells in the bone marrow that are based on the proportion of different components, mononuclear cells adherent culture. Essentially no much difference between the two. With in-depth understanding of MSC surface antigen, and anyone using immunological methods such as flow cytometry, immunofluorescence beads, etc. be isolated and purified. But after flow sorting or magnetic bead cell appeared after some problems such as slow growth, coupled with the high cost and technical difficulty, to some extent limited the widespread application of these methods.
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