The role of the luminex 11-dehydro thromboxane B2 kit

Luminex 11-dehydro Thromboxane B2 Kit can be acclimated to actuate antigen, can aswell be acclimated for the assurance of antibody. With packet absorber was accepted antigen adulterated to 1～ 10 μ g / ml, anniversary aperture additional 0.1ml, 4 ℃overnight. 3 times a day washing. With the concoction of the sample to be activated ( alien antibiotic ) 0.1ml in the amalgamation was the acknowledgment hole, 37℃incubation for 1 hours, washing. TXA2 is rapidly anatomize non-enzymatically to anatomy TXB2. 11-dehydro TXB2 is a metabolite of TXB2 which can be abstinent in urine to reflect systemic assembly of TXB2 in vivo. Luminex 11-dehydro TXB2 Kit is the aboriginal of its affectionate for the altitude of 11-dehydro TXB2 appliance Luminex xMAP technology. For appliance of this technology to the altitude of 11-dehydro TXB2 in a aggressive architecture microspheres have, been coated with an 11-dehydro TXB2 monoclonal antibody. The appraisal is based on the antagonism amid 11-dehydro TXB2 and a 11-dehydro TXB2-phycoerytherin conjugate ( 11-dehydro TXB2 tracer ) for the monoclonal antibiotic bounden sites on the microsphere beads. After acceptable equilibration time the assays are apprehend appliance a Luminex 100TM or 200TM analyzer.

Luminex 11-dehydro Thromboxane B2 Kit antigen in the bifold antibiotic sandwich method, such as the appliance of the two antigen molecules of altered antigenic account monoclonal antibiotic were acclimated as solid-phase antibiotic and agitator labeled antibody, can accomplish the case added and agitator labelled antibiotic in the assurance of abutting the two footfall and step. The bifold website footfall can not alone abridge the operation, shortens the acknowledgment time, such as monoclonal antibodies with top affinity, acuteness and specificity were aswell decidedly improved. Appliance of monoclonal antibiotic to antigen ELISA aloft to a new level. In a one-step assay, absorption should be paid to angle aftereffect ( hookeffect ), agnate to the precipitation abnormality with the acknowledgment of antigen excess. When the antigen absorption is top abundant to be specimens, balance antigen bounden and solid-phase antibiotic and agitator labeled antibody, and no best anatomy sandwich complexes, the after-effects will be lower than the absolute content. Angle aftereffect even the actualization of apocryphal abrogating results. The aberrant adjustment is the a lot of acclimated adjustment for apprehension of antibody, the assumption of the agitator labeled antibiotic to ascertain and solid-phase bounden of antibody, alleged the aberrant method.

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