20S Proteasome Assay Kit characteristics

The 20S Proteasome Assay Kit is the proteolytic amount atom of a ample protein abasement complex, the 26S proteasome. Proteasome inhibitors display anti-inflammatory and antiproliferative effects, affirmation that the proteasome may be an important biologic ambition for the assay for blight and anarchic diseases. 20S Proteasome Assay Kit employs a specific 20S substrate, SUC-LLVY-AMC which, aloft break by the alive enzyme, generates a awful beaming artefact with an discharge amicableness at 480 nm. The kit is simple to use and can be calmly acclimatized to top throughput screening for ameliorative compounds acclimation activation of the 20S proteasome.According to the accepted operation to allegorize the use of protease reagent kit can ascertain 2 ng / ml pronase. Acuteness can be continued through the evolution time and / or advance the evolution temperature increase.

20S Proteasome Assay Kit for the apprehension of biological samples absolute protease action with two altered methods. The two kit can be acclimated to appraise the protease ablution agreement action or on the protein in the sample of protease abuse quantitatively. Kit application amber acerbic casein or fluorescein labelled casein as protease substrate. The substrate can be generated by the hydrolysis of peptides, and the two reagent box appropriately through the apprehension of peptide amino or polypeptide beaming characteristics change of protease test. With kit in trypsin accepted allegory on protease activity, quantitative analysis. Artefact features: no acerb precipitation agent. The agreement in a microtiter bowl in. Compared to the blunt casein protease abstracts are acute 1000 times, 3 times faster, extenuative bisected of the sample. The accomplished agreement performed aural one hour. In the ELISA lath apprehend apparatus for accompanying altitude of assorted samples. By artlessly adjusting the time / temperature /pH can accomplish the best sensitivity.

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