What is Aconitase Assay Kit?

Aconitase Appraisal Kit is an iron-sulfur protein absolute a [Fe4S4]2+ array that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Admitting acknowledgment of aconitase to oxidants renders the agitator inactive, accident of aconitase action in beef or in biological samples advised with pro-oxidants has been interpreted as a admeasurement of oxidative damage.Aconitase Appraisal Kit provides a simple, reproducible, and acute apparatus for assaying aconitase from tissue homogenates or corpuscle lysates. In this assay, citrate is isomerized by aconitase into isocitrate, which is again adapted to α-ketoglutarate in a acknowledgment catalyzed by isocitric dehydrogenase. These reactions are monitored by barometer the access in absorbance at 340 nm associated with the accumulation of NADPH. The amount of NADPH assembly is proportional to aconitase activity.

The Aconitase Appraisal Kit is inhibited by citrate analogs, and fluoracetate. Other inhibitors cover oxidative accent agents such as peroxynitrite, hydrogen achromatize and superoxide, which inactivate the agitator by alteration the [4Fe-4S] to a [3Fe-4S] cluster. Therefore, aconitase is advised a acceptable brand of mitochondrial and cellular oxidative stress. This inhibition of mitochondrial aconitase can advance to a decreased action production, admitting in cytosolic aconitase it triggers bounden of the agitator to mRNA adamant acknowledgment elements consistent in added announcement of adamant uptake proteins and decreased archetype of adamant sequestering protein.A hydroxyl scavenging band-aid (Aconitase canning solution) is supplied with this kit to advance aconitase action during sample preparation. An inactivated [3Fe-4FS] aconitase may be activated in vitro by the accession of adamant and cysteine.

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