11-keto Testosterone EIA Kit description

11-keto Testosterone EIA Kit is a additional key androgenic steroid begin in fish. It occurs in males calm with testosterone in amounts which alter from beneath than 1 ng/ml to as abundant as 50-100 ng/ml, depending on the breed and the date of the changeable cycle. In the sea bass, testosterone concentrations are about college than 11-KT, with aiguille levels begin afterwards the breeding season. 11-KT, on the added hand, charcoal at levels beneath than 1 ng/ml but rises abruptly to 4-6 ng/ml during spermiation at the acme of the breeding season. 11-KT aswell shows alone variations in Arctic char, with ascendant males accepting decidedly college 11-KT levels. The microtiter bowl provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are again added to the adapted microtiter bowl wells with a Horseradish Peroxidase ( HRP ) -conjugated 11-KT and antibiotic alertness specific for 11-KT, and incubated. Again substrate solutions are added to anniversary well.

11-keto Testosterone EIA Kit storage:1. Unopened analysis kits should be stored at 2-8 C aloft cancellation and the microtiter bowl should be kept in a closed bag. The analysis kit may be acclimated throughout the cessation date of the kit, provided it is stored as assigned above. Refer to the amalgamation characterization for the cessation date. 2 Opened analysis bowl should be stored at 2-8 C in the aluminum antithesis bag with desiccants to abbreviate acknowledgment to clammy air. The kits will abide abiding until the expiring date shown, provided it is stored as assigned above. 3 A microtiter bowl clairvoyant with a bandwidth of 10 nm or beneath and an optical body ambit of 0-3 OD or greater at 450nm amicableness is adequate for use in absorbance measurement. Microplate clairvoyant able of barometer absorbance at 450 nm, with the alteration amicableness set at 540 nm or 570 nm. Pipettes and pipette tips. Deionized or distilled water. Squirt bottle, assorted dispenser, or automatic microplate washer. An incubator which can accommodate abiding evolution action S up to 37 °C ± 0.5° C.

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