Yarrowia lipolytica can be used to produce methane from lipids. It assimilates hydrocarbons and produces citric acid from n-alkanes, vegetable oils or glucose under aerobic conditions. Yarrowia lipolytica is routinely isolated from different food media (ie.cheeseflowers, sausages). The tricarboxylic acid cycle has an amphibolic role in metabolism; it functions not only as an oxidative device coupled to energy production but also provides building blocks for the synthesis of important molecules such as porphyrins and several amino acids.
Yarrowia lipolytica LGAM S(7)1 presented remarkable growth on industrial glycerol used as sole carbon substrate. Nitrogen-limited flask cultures were accompanied by restricted synthesis of reserve lipid, whilst amounts of citric acid were produced extracellularly.
On the contrary, high amounts of reserve lipid (up to 3.5 g /l, 43% w/w of lipids in dry biomass) were produced in highly aerated continuous cultures. It is a hemiascomycetous yeast and these yeasts defend a homogenised phylogenetic mathematical group of eukaryotes with a relatively boastfully multifariousness at physical and ecological levels.
Lipid production was favoured at low specific dilution rates whilst fat-free material yield increased over the whole range of D (h?1). The maximum volumetric productivity obtained was 0.12 g lipid/l h. Storage lipid composition did not present remarkable changes in the specific dilution rates tested. Oleate and linoleate were the dominant cellular fatty acids.
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