Wednesday, February 29, 2012

What is Actidione?

Actidione (also known as Cycloheximide) is used as an selective antibiotic. It inhibits the protein synthesis (DNA-dependent RNA) of saprobic fungi eukaryotes. by binding with the 80S ribosome, while inactive against dermatophytes and systemic fungi. But it is not active against prokaryotes. it is used in the selection of wild type genes. Actidione is used as a plant growth regulator to stimulate ethylene production. It is used as a rodenticide and other animal pesticide. Actidione is used as a media to detect to unwanted bacteria by suppressing yeasts and molds in beer fermentation. Some products such as nisin have this effective function as a preservative for food and beverage. But cycloheximide is highly toxic is not suitable for human use as a therapeutic antibiotic. Agricultural application also is decreasing due to the health risks. It is used as an agent effective against psoriasis.
Actidione is an inhibitor of protein biosynthesis in eukaryotic organisms, produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effect by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome) thus blocking translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). It is inexpensive and works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium.
Due to significant toxic side effects, including DNA damage, teratogenesis, and other reproductive effects (including birth defects and toxicity to sperm), cycloheximide is generally used only in in vitro research applications, and is not suitable for human use as a therapeutic compound. Although it has been used as a fungicide in agricultural applications, this application is now decreasing as the health risks have become better understood.
Cycloheximide is degraded by alkali (pH > 7), decontamination of work surfaces and containers can be achieved by washing with a non-harmful alkali solution such as soap.
Since cycloheximide is an effective inhibitor of protein biosynthesis in eukaryotes only, it may be used to distinguish between proteins translated in the mitochondria and proteins translated in the cytosol. mRNA translated in cytosol or ER from mRNA derived from the nucleus will not be expressed in the presence of cycloheximide. Conversely, translation using mitochondrial ribosomes is unaffected by cycloheximide, and mitochondrial genes will continue to be expressed.
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Tuesday, February 28, 2012

What is Kloeckera?

Kloeckera is a mitosporic fungal genus used in the fermentation of wine. Teleomorphs are in the genus HANSENIASPORA.
Description of Kloeckera
Kloeckera apiculata, is a yeast of the asco- mycetes group belonging to the Hemiascomycetes class, Endomycetales order, Saccharomycetaceae.Kloeckera apiculata by glucose mc1 and propose a pathway involved in carbohydrate Xocculation induction.
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Monday, February 27, 2012

What is Hygromycin B used for?

Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.
Hygromycin B was originally developed in the 1950s for use with animals and is still added into swine and chicken feed as an anthelmintic or anti-worming agent (product name: Hygromix). Hygromycin B is produced by Streptomyces hygroscopicus, a bacterium isolated in 1953 from a soil sample. Resistance genes were discovered in the early 1980s. Mechanism of action: Hygromycin is active against both prokaryotic and eukaryotic cells. It acts by inhibiting polypeptide synthesis. It stabilizes the tRNA-ribosomal acceptor site, thereby inhibiting translocation.
Use of Hygromycin B
In the laboratory it is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene. The resistance gene is a kinase that inactivates hygromycin B through phosphorylation. Since the discovery of hygromycin-resistance genes, hygromycin B has become a standard selection antibiotic in gene transfer experiments in many prokaryotic and eukaryotic cells. Based on impurity monitor method, four different kinds of impurities are discovered in commercial hygromycin B from different suppliers and toxicities of different impurities to the cell lines are described in the following external links.
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Sunday, February 26, 2012

What is Pichia?

Pichia (Hansenula and Hyphopichia are obsolete synonyms) is a genus of yeasts in the family Saccharomycetaceae with spherical, elliptical or oblong acuminate cells. Pichia is a teleomorph, and forms during sexual reproduction hat-shaped, hemispherical or round ascospores. The anamorphs of some Pichia species are Candida species. The asexual reproduction is by multilateral budding.
Lactose is neither fermented by this species nor assimilated. The behavior with regard to other carbohydrates is dependent on the different species. Nitrate is always assimilated.
Some Pichia representatives can be found in raw milk and cheese, such as P. anomola (formerly named Hansenula anomala). P. anomala has been shown to combat the undesirable mold Aspergillus flavus, which contaminates food sources such as tree nuts and corn, and produces aflatoxins. Researchers of the Agricultural Research Service found that when pistachio trees were treated with P. anomala the growth of A. flavus was inhibited up to 97%. In addition to inhibiting A. flavus, the yeast may also help protect other agricultural crops from unwanted molds that affect the crop’s taste, texture, yield, and safety. In smeared surface ripened cheese the most important species is P. membranifaciens that also occurs on cream cheese. The formation of a so-called pellicle is typical. Another member of the genus, P. pastoris is widely used in molecular biology and biotechnology as an expression system. P. angusta furthermore called Hansenula polymorpha or H. polymorpha, is a model organism for studying the functions of peroxisomes and their underlying molecular biology.
Some Pichia species (eg P. ohmeri) have recently been clinically been proven to be pathogens, better known as so-called opportunistic pathogens in immunocompromised humans.
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Thursday, February 23, 2012

What is Brecibacterium?

Brecibacterium is a genus of bacteria of the order Actinomycetales. They are Gram-positive soil organisms. It is the sole genus in the family Brevibacteriaceae.
Brecibacterium linens is ubiquitously present on the human skin, where it causes foot odor. The same bacterium is also employed to ferment several cheeses such as Limburger, Port-du-Salut and Năsal. Its smell also attracts mosquitoes.
Brevibacterium genus is a heterogeneous mixture of coryneform organisms that have particular application to industrial production of vitamins, amino acids for fine chemical production, and are commonly used in cheese production. This genus contains 9 species from diverse habitats, such as soil, poultry, fish, human skin, and food. While Brevibacterium linens is phenotypically similar to Arthrobacter globiformis cellular pigmentation, cell wall composition, DNA/DNA hybridization and 5s RNA analysis show that Brevibacterium is distinctly different. PFGE analysis indicates that diversity within the species is related to polymorphisms in the 16S rRNA genes with genome sizes that range from 3.2 and 3.9 Mbp
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Wednesday, February 22, 2012

What is Ubenimex?

Ubenimex (INN), also known as bestatin, is a competitive protease inhibitor. It is an inhibitor of aminopeptidase B, leukotriene A4 hydrolase, aminopeptidase N. It is being studied for use in the treatment of acute myelocytic leukemia
Ubenimex is a competitive aminopeptidase B inhibitor with an IC50 of 100 mg/ml for K562 cells. Proliferation of all the cell lines except KG1 was inhibited by bestatin. P39/TSU, HL60 and U937 were highly sensitive, with 50% growth inhibitory concentrations (IC50) close to the maximum serum concentration when bestatin was orally administered at 30 mg in clinical application. All cell lines except for K562 highly expressed CD13, but a clear correlation between the sensitivity to bestatin and expression of CD13 was not observed. Other aminopeptidase inhibitors such as amastatin A, arphamenine B and WM15 antibody showed no growth inhibitory effects.
Ubenimex is an antitumor agent effective against murine syngeneic tumors including mouse colon 26 and C1498 leukemia. It was active against MNNG-induced rat tumor by oral administration. It inhibits leucine aminopeptidase and aminopeptidase B in cell me
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Tuesday, February 21, 2012

Uses of Micrococcus

Micrococcus is a genus of bacteria in the Micrococcaceae family. Micrococcus occurs in a wide range of environments, including water, dust, and soil. Micrococci have Gram-positive spherical cells ranging from about 0.5 to 3 micrometers in diameter and are typically appear in tetrads.
uses
Micrococcus, like many other representatives of the Actinobacteria, can be catabolically versatile, with the ability to utilize a wide range of unusual substrates, such as pyridine, herbicides, chlorinated biphenyls, and oil. They are likely involved in detoxification or biodegradation of many other environmental pollutants. Other Micrococcus isolates produce various useful products, such as long-chain (C21-C34) aliphatic hydrocarbons for lubricating oils.
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Monday, February 20, 2012

Where to find Polymyxin B Sulphate?

Polymyxin B Sulphate is antibiotic derived from a strain of Bacillus polymyxa. Polymyxin B Sulphate is a sulfate salt of the least toxic member of the polymixin group. Polymyxin B (Sulphate) is bacteriocidal for many gram negative bacteria. Polymyxin B (Sulphate) is a cationic, basic peptide contains fatty acid D-6-methyloctan-1-oic acid and amino acids L-threonine and L-diaminobutyric acid. Polymyxin B Sulphate is used in the treatment of various systemic, urinary tract, otic, ophthalmic and cutaneous infections due to susceptible gram -ve bacteria. Polymyxin B (Sulphate) is administered orally, parentrally and topically, but nowadays, rarely used parentrally due to its potential for causing nephrotoxicity and/or neurotoxicity. Polymyxin B (Sulphate) is more commonly administered as either a topical or otic agent in combination with neomycin or bacitracin. Polymyxin B (Sulphate) is also available in ophthalmic solutions in combination with either trimethoprim B or in combination with bacitracin and neomycin. Polymyxin B is also used in combination with neomycin as a urinary tract irrigant. Polymyxin B was originally approved by the FDA in 1951.
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Sunday, February 19, 2012

What is Lactobacillus fermentum?


Lactobacillus fermentum is a Gram-positive species of bacterium in the genus Lactobacillus. It is associated with active dental caries lesions. It is also commonly found in fermenting animal and plant material. It has been found in sourdough. A few strains are considered probiotic or "friendly" bacteria in animals and at least one strain has been applied to treat urogenital infections in women. Some strains of lactobacilli formerly classified as Lactobacillus fermentum (such as RC-14) have since been reclassified as Lactobacillus reuteri.
Characteristics
Lactobacillus fermentum belongs to the genus Lactobacillus. Species in this genus are used for a wide variety of applications. These applications include food and feed fermentation. It has been found that some strains for Lactobacillus fermentum have natural resistances to certain antibiotics and chemotherapeutics. They are considered potential vectors of antibiotic resistance genes from the environment to humans or animals to humans.
Lactobacillus fermentum can also be a normal inhabitant of the human intestinal tract and some strains have been associated with cholesterol metabolism.
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Thursday, February 16, 2012

What is Pseudomonas?

Pseudomonas is a genus of gammaproteobacteria, belonging to the family Pseudomonadaceae containing 191 validly described species.
Pseudomonas aeruginosa is increasingly recognized as an emerging opportunistic pathogen of clinical relevance. Several different epidemiological studies indicate antibiotic resistance is increasing in clinical isolates.
Use
biocontrol agents, bioremediation agents, Food spoilage agents
As a result of their metabolic diversity, ability to grow at low temperatures and ubiquitous nature, many Pseudomonas spp. can cause food spoilage. Notable examples include dairy spoilage by P. fragi, mustiness in eggs caused by P. taetrolens and P. mudicolens, and P. lundensis, which causes spoilage of milk, cheese, meat, and fish.
Pseudomonas aeruginosa is an opportunistic bacteria that lives in soil, water, and even in environments like hot tubs. For most healthy people, this bacteria seldom poses a problem. Occasionally people will develop conditions like hot tub rash, and swimmer’s ear, which may be due to contact with these germs. These conditions can sometimes resolve without treatment, or with minimal treatment, like antibiotic drops for swimmer’s ear.
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Wednesday, February 15, 2012

What is Lactic acid bacteria as probiotics?

Lactic acid bacteria (LAB) comprise a clade of Gram-positive, low-GC, acid-tolerant, generally non-sporulating, non-respiring rod or cocci that are associated by their common metabolic and physiological characteristics. These bacteria, usually found in decomposing plants and lactic products, produce lactic acid as the major metabolic end-product of carbohydrate fermentation. This trait has, throughout history, linked LAB with food fermentations, as acidification inhibits the growth of spoilage agents. Proteinaceous bacteriocins are produced by several LAB strains and provide an additional hurdle for spoilage and pathogenic microorganisms.
Probiotics
Lactic acid bacteria as probiotics are products aimed at delivering living, potentially beneficial, bacterial cells to the gut ecosystem of humans and other animals, whereas prebiotics are indigestible carbohydrates delivered in food to the large bowel to provide fermentable substrates for selected bacteria. Strains of LAB are the most common microbes employed as probiotics. Two principal kinds of probiotic bacteria, members of the genera Lactobacillus and Bifidobacterium, have been studied in detail.
Most probiotic strains belong to the genus Lactobacillus. Probiotics have been evaluated in research studies in animals and humans with respect to antibiotic-associated diarrhoea, travellers' diarrhoea, pediatric diarrhoea, inflammatory bowel disease, and irritable bowel syndrome. In the future, probiotics possibly will be used for different gastrointestinal diseases, vaginosis, or as delivery systems for vaccines, immunoglobulins, and other therapies.
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Tuesday, February 14, 2012

Geotrichum vs. Geotrichum candidum



Geotrichum is a genus of fungi found worldwide in soil, water, air, and sewage, as well as in plants, cereals, and dairy products; it is also commonly found in normal human flora and is isolated from sputum and feces. It was first described in 1809 by Johann Heinrich Friedrich Link.
The genus Geotrichum includes several species: The most common species is Geotrichum candidum. Geotrichum clavatum and Geotrichum fici are among other Geotrichum species. Geotrichum fici has an intense smell resembling that of pineapple.
Geotrichum candidum is a fungus or mold that can act as a plant pathogen causing sour rot on peach, nectarine, tomato and carrot. It is also widely used in the production of many dairy products including many natural rind cheeses such as Camembert and other bloomy rind cheese, Saint-Nectaire, Tomme de Savoie, and many other cheeses made in those styles. It is also found in the Nordic dairy product viili and is responsible for the velvety surface of the product. One of the leading experts in how this mold affects cheeses during aging is Sister Noella Marcellino of the Abbey of Regina Laudis.
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Where to buy Polymyxin B Sulphate?

CAS No.: 1405-20-5
M.F.: C56H98N16O13. H2SO4
M.W.: 1301.56
DESCRIPTION: WHITE TO ALMOST WHITE POWDER,HYGROSCOPIC.
SOLUBILITY: FREELY SOLUBLE IN WATER;SLIGHTLY SOLUBLE IN ALCOHOL.
IDENTIFICATION
LIQUID CHROMATOGRAPHIC IDENTIFICATION TEST
A REDDISH VIOLET COLOR IS PRODUCED
TEST FOR SULFATE
PH(2%): 5.0~7.0
PH(0.5%): 5.0~7.5
SPECIFIC ROTATION: -78°-  -90°
LOSS ON DRYING: NOT MORE THAN 7.0%
PHENYLALANINE: 9.0%~12.0%
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Sunday, February 12, 2012

What is Yarrowia?

Yarrowia is a fungal genus in the family Dipodascaceae. The genus is monotypic, containing the single species Yarrowia lipolytica, a yeast. It can use unusual carbon sources, such as hydrocarbons. This makes it of industrial interest.
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Thursday, February 9, 2012

Where to buy Nocardia?

Nocardia is a genus of weakly staining Gram-positive, catalase-positive, rod-shaped bacteria. It forms partially acid-fast beaded branching filaments (acting as fungi, but being truly bacteria). It has a total of 85 species. Some species are non-pathogenic while others are responsible for nocardiosis. Nocardia are found worldwide in soil that is rich with organic matter.
Treatment
Antibiotic therapy with a sulfonamide, most commonly trimethoprim-sulfamethoxazole, is the treatment of choice. People who take trimethoprim-sulfamethoxazole for other reasons, such as prevention of Pneumocystis jirovecii infection, appear to have fewer Nocardia infections, although this protective effect has been considered unreliable and some studies have disputed it altogether.
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Tuesday, February 7, 2012

Specifications of Enterobacter

Enterobacter is a genus of common Gram-negative, facultatively-anaerobic, rod-shaped bacteria of the family Enterobacteriaceae. Several strains of the these bacteria are pathogenic and cause opportunistic infections in immunocompromised hosts and in those who are on mechanical ventilation.
Treatment
1. Fourth generation of cephalosporin,a β-lactam antibiotic Cefepime 2. Imipenem (carbapenems) is of choice in Enterobacter treatment.
3. Aminoglycosides such as amikacin have been found very effective as well.
4. Quinolones are an effective alternative.
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How to Use Ethyl levulinate

Ethyl levulinate is a colourless to pale yellow liquid with apple odour.
Sulfonic acid-functionalized ionic liquids are charismatic and foretelling catalyst for the conversion of sugars to ethyl levulinate and ethyl-D-glucopyranoside in ethanol.
These task-specific ionic liquids can be recovered and reused in at lowest three cycles/seconds in the conversion of fructose to ethyl levulinate without any loss of activity.
Sulfonic acid functionalised SBA-15 (SO3H-SBA-15), sulfated zirconia and beta, Y, ZSM-5 and mordenite zeolite catalysts have been applied for the dehydration of sugars to ethyl levulinate and ethyl-d-glucopyranoside (EDGP) using ethanol as solvent and reactant. The SO3H-SBA-15 catalyst showed a high catalytic activity for the selective conversion of fructose to ethyl levulinate (57%) and glucose to EDGP (80%) at 140 °C, whereas the disaccharide sucrose gave way a earthshaking sum of money of both products. The SO3H-SBA-15 catalysts were found to be highly active compared to the zeolites under identical reaction conditions.
Ethyl levulinate is also called      Levulinic acid ethyl ester 4-oxopentanoate; 4-Oxopentanoic acid ethyl ester; Ethyl laevulinate; Ethyl Ethyl 4-oxovalerate; Ethyl 4-ketovalerate; Ethyl 3-acetylpropionate.
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Monday, February 6, 2012

What is Baicllus Used for

Bacillus is a genus of Gram-positive, baculiform bacteria and a member of the division Firmicutes. Bacillus species can be obligate aerobes or facultative anaerobes, and test positive for the enzyme catalase. Ubiquitous in nature, Bacillus includes both free-living and pathogenic species.
Bacillus thuringiensis (Bt) is an insecticide with fantastical dimensions that come through utile for pest control in certain situations. Bt is a naturally occurring bacterium common in soils throughout the world. Several strains can infect and kill insects. Because of this property, Bt has been developed for insect control. At confront, Bt costs the only "microbial insecticide" in widespread use.
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What is Baicllus Used for

Bacillus is a genus of Gram-positive, baculiform bacteria and a member of the division Firmicutes. Bacillus species can be obligate aerobes or facultative anaerobes, and test positive for the enzyme catalase. Ubiquitous in nature, Bacillus includes both free-living and pathogenic species.
Bacillus thuringiensis (Bt) is an insecticide with fantastical dimensions that come through utile for pest control in certain situations. Bt is a naturally occurring bacterium common in soils throughout the world. Several strains can infect and kill insects. Because of this property, Bt has been developed for insect control. At confront, Bt costs the only "microbial insecticide" in widespread use.
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What is Baicllus Used for

Bacillus is a genus of Gram-positive, baculiform bacteria and a member of the division Firmicutes. Bacillus species can be obligate aerobes or facultative anaerobes, and test positive for the enzyme catalase. Ubiquitous in nature, Bacillus includes both free-living and pathogenic species.
Bacillus thuringiensis (Bt) is an insecticide with fantastical dimensions that come through utile for pest control in certain situations. Bt is a naturally occurring bacterium common in soils throughout the world. Several strains can infect and kill insects. Because of this property, Bt has been developed for insect control. At confront, Bt costs the only "microbial insecticide" in widespread use.
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What is Baicllus Used for

Bacillus is a genus of Gram-positive, baculiform bacteria and a member of the division Firmicutes. Bacillus species can be obligate aerobes or facultative anaerobes, and test positive for the enzyme catalase. Ubiquitous in nature, Bacillus includes both free-living and pathogenic species.
Bacillus thuringiensis (Bt) is an insecticide with fantastical dimensions that come through utile for pest control in certain situations. Bt is a naturally occurring bacterium common in soils throughout the world. Several strains can infect and kill insects. Because of this property, Bt has been developed for insect control. At confront, Bt costs the only "microbial insecticide" in widespread use.
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Thursday, February 2, 2012

The Applications of Acremonium

Acremonium species are usually slow growing and are initially compact and moist. Acremonium hyphae are fine and hyaline and produce mostly simple phialides.
Their conidia are usually one-celled (i.e. ameroconidia), hyaline or pigmented, globose to tube-shaped, and for the most part aggregated inwards slimy heads at the apex of each phialide.
The genus Acremonium includes some of the most simply structured of all filamentous anamorphic fungi. The characteristic morphology consists of septate hyphae giving rise to thin, tapered, mostly lateral phialides produced singly or in small groups. Conidia tend to be unicellular, produced in mucoid heads or unconnected chains. They can be hyaline or melanised, but the hyphae are usually hyaline.
Acremonium alcalophilum is the only known cellulolytic fungus that thrives in alkaline conditions that can be cultured readily in the laboratory. This fungus was isolated from sludge of pig manure compost. Its optimal conditions for growth are 30°C and pH 9.0-9.2. Examines on-duty bacteria and fungi that can tolerate a across-the-board pH cooking stove indicate that adaptation to high up pH conditions involves products that function in the extracellular neighborhood or at the cellular telephone boundary. Hence the genome sequence of A. alcalophilum is expected to provide a rich genetic resource for identifying extracellular enzymes with pH optima in the alkaline range.
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The New Information about Aeromonas

Aeromonas is a gram-negative, facultative anaerobic rod that morphologically resembles members of the family Enterobacteriaceae. Fourteen species of Aeromonas have been described, most of which have been associated with human diseases. The most important pathogens are A. hydrophila, A. caviae, and A. veronii biovar sobria. The organisms are ubiquitous in fresh and brackish water.
Aeromonas is a genus of bacteria that is ubiquitous in the environment. It is exhibit in totally eccentrics of water supply worldwide as well as food and soil. These organisms have also been isolated from a variety of foods, including red meats (beef, pork, lamb), poultry, produce, fish, and shellfish. There are approximately 16 contrasting species inwards this genus, the most beneficial known of which is Aeromonas hydrophila. Physiologically, Aeromonas are standardized to bacteria fashionable the coliform chemical group and can be isolated from similar environments.
Aeromonas is a normal part of the microflora found in these waters, and its presence does not indicate the water has been polluted. The cytotoxic enterotoxin gene (act), a single-chain polypeptide, is one of the primary genes that makes this species pathogenic. Heat-labile cytotonic enterotoxins (elevation), a heat-stable cytotonic enterotoxins (Ast) also have genes that contribute to toxicity. These are labeled alt and ast, respectively. Albert et. al. (2000) noted that three distinct genes code for these enterotoxins: act, alt and ast.
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Wednesday, February 1, 2012

The Functions of Penicillium

Penicillium (from Latin penicillus: paintbrush) is a genus of ascomycetous fungi of major importance in the environment, food and drug production. It produces penicillin, a molecule that is used as an antibiotic, which drinks down or boodle the growth of certain kinds of bacteria in spite of appearance the consistence. Decisions looking at the propriety of any limited procedure, treatment, or drug dosage must be made by the patient's physician afterwards considerateness of entirely of the relevant facts regarding the individual patient.
Penicillium species are very common models. Just about 200 species have been described. They are commonly called the blue or green moulds because they bring forth enormous measures from light-green, blueish or yellowish spores which give them their characteristic colours. Critical review of the manufacturing business intersection information or consultation with a pharamacist is advised if you are uncertain about the potential drug-drug interactions of an agent. Spores of Penicillium are constitute everywhere fashionable the atmosphere and soil. Penicillium species are one of the most common causes of spoilage of fruits and vegetables.
Penicillium roqueforti is a common saprophytic fungus, that is widespread in nature and can be isolated from soil, decaying organic substances and plant parts. The major industrial uses of this fungus are for the production of blue cheeses, seasoner factors, bactericides, polysaccharides, proteinases and extra enzymes.
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